CpG stimulation of chronic lymphocytic leukemia cells induces a polarized cell shape and promotes migration in vitro and in vivo.

CpG stimulation of chronic lymphocytic leukemia cells induces a polarized cell shape and promotes migration in vitro and in vivo.

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In order to accomplish their physiological functions leukocytes have the capability to migrate.As a prerequisite they need to adopt a polarized cell shape, forming a leading edge at the front and a uropod at rear pole.In this study we explored the capability of chronic lymphocytic leukaemia (CLL) cells to adopt this leukocyte-specific migration phenotype.Furthermore, we studied Bikini bottoms the impact of the Toll-like receptor 9 (TLR9) agonists CpGs type A, B and C and the antagonist oligodesoxynucleotide (ODN) INH-18 on the cell polarization and migration process of primary human CLL cells.

Upon cultivation, a portion of purified CLL cells adopted polarized cell shapes spontaneously (range 10-38%).Stimulation with CpG ODNs type B (ODN 2006) and CpGs type C (ODN 2395) significantly increased the frequency of morphologically polarized CLL cells, while ODN INH-18 was hardly able to act antagonistically.Like in human hematopoietic stem and progenitor cells, in morphologically 7 Piece Outdoor Sectional polarized CLL cells CXCR4 was redistributed to the leading edge and CD50 to the uropod.Coupled to the increased frequencies of morphologically polarized cells, CpGs type B and C stimulated CLL cells showed higher migration activities in vitro and following intravenous injection higher homing frequencies to the bone marrow of immunocompromised NOD.

Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice.Thus, presumably independent of TLR-9 signaling, CpGs type B and C promote the cellular polarization process of CLL cells and their ability to migrate in vitro and in vivo.